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polyclonal rabbit anti-mouse/human sr-bi antibody  (Novus Biologicals)


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    Structured Review

    Novus Biologicals polyclonal rabbit anti-mouse/human sr-bi antibody
    Polyclonal Rabbit Anti Mouse/Human Sr Bi Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+mouse+human+sr+bi+antibody/rabbit+polyclonal+antibody/pmc04530130-208-61-66
    Average 90 stars, based on 1 article reviews
    polyclonal rabbit anti-mouse/human sr-bi antibody - by Bioz Stars, 2026-09
    90/100 stars

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    Article Title: Imaging macular carotenoids and their related proteins in the human retina with confocal resonance Raman and fluorescence microscopy.
    Article Snippet: Lutein and zeaxanthin are highly concentrated at the central region of the human retina, forming a distinct yellow spot known as the macula lutea.. The delivery and retention of the macular pigment carotenoids in the macula lutea involves many proteins, but their exact roles remain incompletely understood.. In our study, we examined the distribution of the twelve known macular carotenoid-related proteins within the human macula and the underlying retinal pigment epithelium (RPE) using both fluorescence and Raman modes on our confocal resonance Raman microscope.

    Article Title: Receptor-Mediated ER Export of Lipoproteins Controls Lipid Homeostasis in Mice and Humans.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Lenti-CRISPR V2 Cas9 D10A This paper N/A pX602 Addgene #61593 pX602-AAV-Cre-SgRNA This paper N/A AAV-TBG-FLAG-hSURF4 This paper N/A AAV-TBG-FLAG-hSURF4 AAA This paper N/A AAV-TBG-GFP Addgene #105535 AAV-TBG-CRE This paper N/A AAV-hPCSK9 D374Y Addgene #58379 Chemicals, Peptides, and Recombinant Proteins BODIPY 493/503 Thermo Fisher Cat#D3922; ANTI-FLAG M2 Affinity Gel Sigma-Aldrich Cat#A2220; Streptavidin-Agarose Sigma-Aldrich Cat#S1638 Succinyl WGA Gel -, Immobilized Lectin EY Laboratories Cat#a-2102-5 Agarose bound Concanavalin A (Con A) Vectorlabs Cat#AL-1003-10 Agarose bound Sambucus Nigra Lectin (SNA, EBL) Vectorlabs Cat#AL-1303 Protease inhibitor Roche Cat#4693132001 TRIzol Reagent Thermo Fisher Cat#15596018 M-MLV Reverse Transcriptase Thermo Fisher Cat#28025013 RNASEOUT Thermo Fisher Cat#10777019 KOD FX Toyobo Cat#KFX-101 T7 Endonuclease I NEB Cat#M0302 Digitonin Sigma-Aldrich Cat#D141 Guanosine 50-triphosphate sodium Sigma-Aldrich Cat#G8877 GTPrS Sigma-Aldrich Cat#G8634 Adenesine5-triphosphate disodium Sigma-Aldrich Cat#A2383 Creatine phosphokinase Roche Cat#C3755 Creatine phosphate Roche Cat#10621714001 Tyloxapol Sigma Cat#T8761 Glucose Sigma Cat#G7528 Insulin Sigma Cat#I0516 SPLASH LIPIDOMIX Mass Spec Standard Avanti Cat#330707 Critical Commercial Assays Serum Triglyceride Determination KIT Sigma-Aldrich Cat#TR0100 CHO kit Zhongshengbeikong Cat#000180 ALT/GPT kit Zhongshengbeikong Cat#000000010 AST/GOT kit Zhongshengbeikong Cat#000000020 BCA Protein Assay Kit Pierce Thermo-Pierce Cat#23227 Silver Stain kit for Mass Spectrometry Life technologies Cat# 24600 Dual-Luciferase Reporter Assay System Promega Cat# E1910 TIANamp Genomic DNA kit TIANGEN Cat# DP304-03 SuperReal PreMix Plus(SYBR Green) TIANGEN Cat# FP205 Experimental Models: Cell Lines HEK293A Thermo Fisher R70507 HEK293T ATCC CRL-3216, RRID:CVCL_0063 Huh7 JCRB Cell Ban Cat# JCRB0403, RRID:CVCL_0336 HepG2 ATCC Cat# HB-8065, RRID:CVCL_0027 Huh7 DSNP line1 This paper N/A (Continued on next page) Cell Metabolism 33, 1–17.e1–e7, January 5, 2021 e2

    Article Title: Cholesterol uptake and efflux are impaired in human trophoblast cells from pregnancies with maternal supraphysiological hypercholesterolemia
    Article Snippet: Proteins were separated as described by polyacrylamide gel electrophoresis in denaturing and reducing conditions, transferred to polyvinylidene difluoride membranes and later probed with primary rabbit polyclonal anti-LDLR, anti-apolipoprotein B 100 (ApoB), anti- apolipoprotein A-I (ApoA-I) (Abcam, UK), anti-SR-BI, anti-ABCA1 and anti-ABCG1 (Novus Biological, USA), (1:1000, 18 h, 4 °C) and mouse monoclonal anti-3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR, 1:500, 18 h, 4 °C) (Santa Cruz Biotechnology, USA) and anti-β-actin (1:5000, 1 h, room temperature) (Sigma-Aldrich, USA) antibodies.

    Article Title: Evidence for hypoxia‐induced dysregulated cholesterol homeostasis in preeclampsia: Insights into the mechanisms from human placental cells and tissues
    Article Snippet: The protein for placental tissue was probed with primary rabbit polyclonal anti- scavenger receptor class B type 1 (SR- BI) (1 μg/mL), anti- ABCG1 (1 μg/mL) (Novus Biological, USA), anti- 3- hydroxy- 3- methylglutaryl- CoA synthase 1 (HMGCS) (1 μg/mL), and anti- 3- hydroxy- 3- methylglutaryl- CoA reductase (HMGCR) (1 μg/mL) (Thermo Fisher Scientific, USA), as well as mouse monoclonal anti- ABCA1 (1 μg/mL) and antiglyceraldehyde 3- phosphate dehydrogenase (GAPDH) (0.2 μg/mL) (Santa Cruz Biotechnology Inc., USA) (18 h, 4°C).

    Electrophoresis:

    Article Title: Cholesteryl Hemiazelate Present in Cardiovascular Disease Patients Causes Lysosome Dysfunction in Murine Fibroblasts.
    Article Snippet: .. After electrophoresis, proteins were transferred toward an activated PVDF membrane in 1x transfer buffer at 4 ◦C for 1 h. Membranes were blocked with blocking buffer (5% BSA or milk in 1X TBS-T–Tris-buffered saline, 0.01% tween) for 1 h at room temperature (RT), followed by overnight incubation at 4 ◦C with the following primary antibodies diluted in blocking buffer: rat anti-LAMP1 (Hybridoma Bank, 1D4B), rabbit anti-CTSD (Abcam, ab75852), rabbit anti-LAL (Abcam, Cambridge, UK, ab154356), mouse anti-P62 (Abgent, San Diego, CA, USA, AP2183b), rabbit anti-LC3B (Cell Signaling, Danvers, MA, USA, 2775S), rabbit Cells 2023, 12, 2826 4 of 19 anti-phospho-mTOR (Cell Signaling, 5536S), rabbit anti-mTOR (Cell Signaling, 2983S), 4E-BP1 (Cell Signaling, 9644S), phosphor-4E-BP1 (Cell Signaling, 2855S), rabbit anti-S6 ribosomal protein (Cell Signaling, 2217S), rabbit anti-phospho-S6 (Cell Signaling, 4858S), rabbit anti-caspase 3 (Cell Signaling, 9661S), rabbit anti-p16-INK4A (Proteintech, Rosemont, IL, USA, 10883-1-AP), mouse anti-p21 (Santa Cruz, Dallas, Texas, United States, sc-6246), mouse anti-SQSTM1 (Abgent, AP2183b), mouse anti-CD36 (BD Biosciences, San Jose, CA, USA, 552544), rabbit anti-SR-BI (NOVUS Biologicals, Littleton, CO, USA, NB400113), rabbit anti-CD204 (Abcam, ab151707), TLR4 (Invitrogen, Carlsbad, CA, USA, 48- 2300), goat anti-GAPDH (SICGEN, AB0049-20), goat anti-calnexin (SICGEN, Cantanhede, Portugal, AB0041-20), mouse anti-β-tubulin (Hybridoma Bank, Iowa City, IA, USA, E7). ..

    Article Title: Cholesteryl Hemiazelate Present in Cardiovascular Disease Patients Causes Lysosome Dysfunction in Murine Fibroblasts
    Article Snippet: .. After electrophoresis, proteins were transferred toward an activated PVDF membrane in 1x transfer buffer at 4 °C for 1 h. Membranes were blocked with blocking buffer (5% BSA or milk in 1X TBS-T–Tris-buffered saline, 0.01% tween) for 1 h at room temperature (RT), followed by overnight incubation at 4 °C with the following primary antibodies diluted in blocking buffer: rat anti-LAMP1 (Hybridoma Bank, 1D4B), rabbit anti-CTSD (Abcam, ab75852), rabbit anti-LAL (Abcam, Cambridge, UK, ab154356), mouse anti-P62 (Abgent, San Diego, CA, USA, AP2183b), rabbit anti-LC3B (Cell Signaling, Danvers, MA, USA, 2775S), rabbit anti-phospho-mTOR (Cell Signaling, 5536S), rabbit anti-mTOR (Cell Signaling, 2983S), 4E-BP1 (Cell Signaling, 9644S), phosphor-4E-BP1 (Cell Signaling, 2855S), rabbit anti-S6 ribosomal protein (Cell Signaling, 2217S), rabbit anti-phospho-S6 (Cell Signaling, 4858S), rabbit anti-caspase 3 (Cell Signaling, 9661S), rabbit anti-p16-INK4A (Proteintech, Rosemont, IL, USA, 10883-1-AP), mouse anti-p21 (Santa Cruz, Dallas, Texas, United States, sc-6246), mouse anti-SQSTM1 (Abgent, AP2183b), mouse anti-CD36 (BD Biosciences, San Jose, CA, USA, 552544), rabbit anti-SR-BI (NOVUS Biologicals, Littleton, CO, USA, NB400-113), rabbit anti-CD204 (Abcam, ab151707), TLR4 (Invitrogen, Carlsbad, CA, USA, 48-2300), goat anti-GAPDH (SICGEN, AB0049-20), goat anti-calnexin (SICGEN, Cantanhede, Portugal, AB0041-20), mouse anti-β-tubulin (Hybridoma Bank, Iowa City, IA, USA, E7). ..

    Membrane:

    Article Title: Cholesteryl Hemiazelate Present in Cardiovascular Disease Patients Causes Lysosome Dysfunction in Murine Fibroblasts.
    Article Snippet: .. After electrophoresis, proteins were transferred toward an activated PVDF membrane in 1x transfer buffer at 4 ◦C for 1 h. Membranes were blocked with blocking buffer (5% BSA or milk in 1X TBS-T–Tris-buffered saline, 0.01% tween) for 1 h at room temperature (RT), followed by overnight incubation at 4 ◦C with the following primary antibodies diluted in blocking buffer: rat anti-LAMP1 (Hybridoma Bank, 1D4B), rabbit anti-CTSD (Abcam, ab75852), rabbit anti-LAL (Abcam, Cambridge, UK, ab154356), mouse anti-P62 (Abgent, San Diego, CA, USA, AP2183b), rabbit anti-LC3B (Cell Signaling, Danvers, MA, USA, 2775S), rabbit Cells 2023, 12, 2826 4 of 19 anti-phospho-mTOR (Cell Signaling, 5536S), rabbit anti-mTOR (Cell Signaling, 2983S), 4E-BP1 (Cell Signaling, 9644S), phosphor-4E-BP1 (Cell Signaling, 2855S), rabbit anti-S6 ribosomal protein (Cell Signaling, 2217S), rabbit anti-phospho-S6 (Cell Signaling, 4858S), rabbit anti-caspase 3 (Cell Signaling, 9661S), rabbit anti-p16-INK4A (Proteintech, Rosemont, IL, USA, 10883-1-AP), mouse anti-p21 (Santa Cruz, Dallas, Texas, United States, sc-6246), mouse anti-SQSTM1 (Abgent, AP2183b), mouse anti-CD36 (BD Biosciences, San Jose, CA, USA, 552544), rabbit anti-SR-BI (NOVUS Biologicals, Littleton, CO, USA, NB400113), rabbit anti-CD204 (Abcam, ab151707), TLR4 (Invitrogen, Carlsbad, CA, USA, 48- 2300), goat anti-GAPDH (SICGEN, AB0049-20), goat anti-calnexin (SICGEN, Cantanhede, Portugal, AB0041-20), mouse anti-β-tubulin (Hybridoma Bank, Iowa City, IA, USA, E7). ..

    Article Title: Cholesteryl Hemiazelate Present in Cardiovascular Disease Patients Causes Lysosome Dysfunction in Murine Fibroblasts
    Article Snippet: .. After electrophoresis, proteins were transferred toward an activated PVDF membrane in 1x transfer buffer at 4 °C for 1 h. Membranes were blocked with blocking buffer (5% BSA or milk in 1X TBS-T–Tris-buffered saline, 0.01% tween) for 1 h at room temperature (RT), followed by overnight incubation at 4 °C with the following primary antibodies diluted in blocking buffer: rat anti-LAMP1 (Hybridoma Bank, 1D4B), rabbit anti-CTSD (Abcam, ab75852), rabbit anti-LAL (Abcam, Cambridge, UK, ab154356), mouse anti-P62 (Abgent, San Diego, CA, USA, AP2183b), rabbit anti-LC3B (Cell Signaling, Danvers, MA, USA, 2775S), rabbit anti-phospho-mTOR (Cell Signaling, 5536S), rabbit anti-mTOR (Cell Signaling, 2983S), 4E-BP1 (Cell Signaling, 9644S), phosphor-4E-BP1 (Cell Signaling, 2855S), rabbit anti-S6 ribosomal protein (Cell Signaling, 2217S), rabbit anti-phospho-S6 (Cell Signaling, 4858S), rabbit anti-caspase 3 (Cell Signaling, 9661S), rabbit anti-p16-INK4A (Proteintech, Rosemont, IL, USA, 10883-1-AP), mouse anti-p21 (Santa Cruz, Dallas, Texas, United States, sc-6246), mouse anti-SQSTM1 (Abgent, AP2183b), mouse anti-CD36 (BD Biosciences, San Jose, CA, USA, 552544), rabbit anti-SR-BI (NOVUS Biologicals, Littleton, CO, USA, NB400-113), rabbit anti-CD204 (Abcam, ab151707), TLR4 (Invitrogen, Carlsbad, CA, USA, 48-2300), goat anti-GAPDH (SICGEN, AB0049-20), goat anti-calnexin (SICGEN, Cantanhede, Portugal, AB0041-20), mouse anti-β-tubulin (Hybridoma Bank, Iowa City, IA, USA, E7). ..

    Blocking Assay:

    Article Title: Cholesteryl Hemiazelate Present in Cardiovascular Disease Patients Causes Lysosome Dysfunction in Murine Fibroblasts.
    Article Snippet: .. After electrophoresis, proteins were transferred toward an activated PVDF membrane in 1x transfer buffer at 4 ◦C for 1 h. Membranes were blocked with blocking buffer (5% BSA or milk in 1X TBS-T–Tris-buffered saline, 0.01% tween) for 1 h at room temperature (RT), followed by overnight incubation at 4 ◦C with the following primary antibodies diluted in blocking buffer: rat anti-LAMP1 (Hybridoma Bank, 1D4B), rabbit anti-CTSD (Abcam, ab75852), rabbit anti-LAL (Abcam, Cambridge, UK, ab154356), mouse anti-P62 (Abgent, San Diego, CA, USA, AP2183b), rabbit anti-LC3B (Cell Signaling, Danvers, MA, USA, 2775S), rabbit Cells 2023, 12, 2826 4 of 19 anti-phospho-mTOR (Cell Signaling, 5536S), rabbit anti-mTOR (Cell Signaling, 2983S), 4E-BP1 (Cell Signaling, 9644S), phosphor-4E-BP1 (Cell Signaling, 2855S), rabbit anti-S6 ribosomal protein (Cell Signaling, 2217S), rabbit anti-phospho-S6 (Cell Signaling, 4858S), rabbit anti-caspase 3 (Cell Signaling, 9661S), rabbit anti-p16-INK4A (Proteintech, Rosemont, IL, USA, 10883-1-AP), mouse anti-p21 (Santa Cruz, Dallas, Texas, United States, sc-6246), mouse anti-SQSTM1 (Abgent, AP2183b), mouse anti-CD36 (BD Biosciences, San Jose, CA, USA, 552544), rabbit anti-SR-BI (NOVUS Biologicals, Littleton, CO, USA, NB400113), rabbit anti-CD204 (Abcam, ab151707), TLR4 (Invitrogen, Carlsbad, CA, USA, 48- 2300), goat anti-GAPDH (SICGEN, AB0049-20), goat anti-calnexin (SICGEN, Cantanhede, Portugal, AB0041-20), mouse anti-β-tubulin (Hybridoma Bank, Iowa City, IA, USA, E7). ..

    Article Title: Cholesteryl Hemiazelate Present in Cardiovascular Disease Patients Causes Lysosome Dysfunction in Murine Fibroblasts
    Article Snippet: .. After electrophoresis, proteins were transferred toward an activated PVDF membrane in 1x transfer buffer at 4 °C for 1 h. Membranes were blocked with blocking buffer (5% BSA or milk in 1X TBS-T–Tris-buffered saline, 0.01% tween) for 1 h at room temperature (RT), followed by overnight incubation at 4 °C with the following primary antibodies diluted in blocking buffer: rat anti-LAMP1 (Hybridoma Bank, 1D4B), rabbit anti-CTSD (Abcam, ab75852), rabbit anti-LAL (Abcam, Cambridge, UK, ab154356), mouse anti-P62 (Abgent, San Diego, CA, USA, AP2183b), rabbit anti-LC3B (Cell Signaling, Danvers, MA, USA, 2775S), rabbit anti-phospho-mTOR (Cell Signaling, 5536S), rabbit anti-mTOR (Cell Signaling, 2983S), 4E-BP1 (Cell Signaling, 9644S), phosphor-4E-BP1 (Cell Signaling, 2855S), rabbit anti-S6 ribosomal protein (Cell Signaling, 2217S), rabbit anti-phospho-S6 (Cell Signaling, 4858S), rabbit anti-caspase 3 (Cell Signaling, 9661S), rabbit anti-p16-INK4A (Proteintech, Rosemont, IL, USA, 10883-1-AP), mouse anti-p21 (Santa Cruz, Dallas, Texas, United States, sc-6246), mouse anti-SQSTM1 (Abgent, AP2183b), mouse anti-CD36 (BD Biosciences, San Jose, CA, USA, 552544), rabbit anti-SR-BI (NOVUS Biologicals, Littleton, CO, USA, NB400-113), rabbit anti-CD204 (Abcam, ab151707), TLR4 (Invitrogen, Carlsbad, CA, USA, 48-2300), goat anti-GAPDH (SICGEN, AB0049-20), goat anti-calnexin (SICGEN, Cantanhede, Portugal, AB0041-20), mouse anti-β-tubulin (Hybridoma Bank, Iowa City, IA, USA, E7). ..

    Saline:

    Article Title: Cholesteryl Hemiazelate Present in Cardiovascular Disease Patients Causes Lysosome Dysfunction in Murine Fibroblasts.
    Article Snippet: .. After electrophoresis, proteins were transferred toward an activated PVDF membrane in 1x transfer buffer at 4 ◦C for 1 h. Membranes were blocked with blocking buffer (5% BSA or milk in 1X TBS-T–Tris-buffered saline, 0.01% tween) for 1 h at room temperature (RT), followed by overnight incubation at 4 ◦C with the following primary antibodies diluted in blocking buffer: rat anti-LAMP1 (Hybridoma Bank, 1D4B), rabbit anti-CTSD (Abcam, ab75852), rabbit anti-LAL (Abcam, Cambridge, UK, ab154356), mouse anti-P62 (Abgent, San Diego, CA, USA, AP2183b), rabbit anti-LC3B (Cell Signaling, Danvers, MA, USA, 2775S), rabbit Cells 2023, 12, 2826 4 of 19 anti-phospho-mTOR (Cell Signaling, 5536S), rabbit anti-mTOR (Cell Signaling, 2983S), 4E-BP1 (Cell Signaling, 9644S), phosphor-4E-BP1 (Cell Signaling, 2855S), rabbit anti-S6 ribosomal protein (Cell Signaling, 2217S), rabbit anti-phospho-S6 (Cell Signaling, 4858S), rabbit anti-caspase 3 (Cell Signaling, 9661S), rabbit anti-p16-INK4A (Proteintech, Rosemont, IL, USA, 10883-1-AP), mouse anti-p21 (Santa Cruz, Dallas, Texas, United States, sc-6246), mouse anti-SQSTM1 (Abgent, AP2183b), mouse anti-CD36 (BD Biosciences, San Jose, CA, USA, 552544), rabbit anti-SR-BI (NOVUS Biologicals, Littleton, CO, USA, NB400113), rabbit anti-CD204 (Abcam, ab151707), TLR4 (Invitrogen, Carlsbad, CA, USA, 48- 2300), goat anti-GAPDH (SICGEN, AB0049-20), goat anti-calnexin (SICGEN, Cantanhede, Portugal, AB0041-20), mouse anti-β-tubulin (Hybridoma Bank, Iowa City, IA, USA, E7). ..

    Article Title: Cholesteryl Hemiazelate Present in Cardiovascular Disease Patients Causes Lysosome Dysfunction in Murine Fibroblasts
    Article Snippet: .. After electrophoresis, proteins were transferred toward an activated PVDF membrane in 1x transfer buffer at 4 °C for 1 h. Membranes were blocked with blocking buffer (5% BSA or milk in 1X TBS-T–Tris-buffered saline, 0.01% tween) for 1 h at room temperature (RT), followed by overnight incubation at 4 °C with the following primary antibodies diluted in blocking buffer: rat anti-LAMP1 (Hybridoma Bank, 1D4B), rabbit anti-CTSD (Abcam, ab75852), rabbit anti-LAL (Abcam, Cambridge, UK, ab154356), mouse anti-P62 (Abgent, San Diego, CA, USA, AP2183b), rabbit anti-LC3B (Cell Signaling, Danvers, MA, USA, 2775S), rabbit anti-phospho-mTOR (Cell Signaling, 5536S), rabbit anti-mTOR (Cell Signaling, 2983S), 4E-BP1 (Cell Signaling, 9644S), phosphor-4E-BP1 (Cell Signaling, 2855S), rabbit anti-S6 ribosomal protein (Cell Signaling, 2217S), rabbit anti-phospho-S6 (Cell Signaling, 4858S), rabbit anti-caspase 3 (Cell Signaling, 9661S), rabbit anti-p16-INK4A (Proteintech, Rosemont, IL, USA, 10883-1-AP), mouse anti-p21 (Santa Cruz, Dallas, Texas, United States, sc-6246), mouse anti-SQSTM1 (Abgent, AP2183b), mouse anti-CD36 (BD Biosciences, San Jose, CA, USA, 552544), rabbit anti-SR-BI (NOVUS Biologicals, Littleton, CO, USA, NB400-113), rabbit anti-CD204 (Abcam, ab151707), TLR4 (Invitrogen, Carlsbad, CA, USA, 48-2300), goat anti-GAPDH (SICGEN, AB0049-20), goat anti-calnexin (SICGEN, Cantanhede, Portugal, AB0041-20), mouse anti-β-tubulin (Hybridoma Bank, Iowa City, IA, USA, E7). ..

    Incubation:

    Article Title: Cholesteryl Hemiazelate Present in Cardiovascular Disease Patients Causes Lysosome Dysfunction in Murine Fibroblasts.
    Article Snippet: .. After electrophoresis, proteins were transferred toward an activated PVDF membrane in 1x transfer buffer at 4 ◦C for 1 h. Membranes were blocked with blocking buffer (5% BSA or milk in 1X TBS-T–Tris-buffered saline, 0.01% tween) for 1 h at room temperature (RT), followed by overnight incubation at 4 ◦C with the following primary antibodies diluted in blocking buffer: rat anti-LAMP1 (Hybridoma Bank, 1D4B), rabbit anti-CTSD (Abcam, ab75852), rabbit anti-LAL (Abcam, Cambridge, UK, ab154356), mouse anti-P62 (Abgent, San Diego, CA, USA, AP2183b), rabbit anti-LC3B (Cell Signaling, Danvers, MA, USA, 2775S), rabbit Cells 2023, 12, 2826 4 of 19 anti-phospho-mTOR (Cell Signaling, 5536S), rabbit anti-mTOR (Cell Signaling, 2983S), 4E-BP1 (Cell Signaling, 9644S), phosphor-4E-BP1 (Cell Signaling, 2855S), rabbit anti-S6 ribosomal protein (Cell Signaling, 2217S), rabbit anti-phospho-S6 (Cell Signaling, 4858S), rabbit anti-caspase 3 (Cell Signaling, 9661S), rabbit anti-p16-INK4A (Proteintech, Rosemont, IL, USA, 10883-1-AP), mouse anti-p21 (Santa Cruz, Dallas, Texas, United States, sc-6246), mouse anti-SQSTM1 (Abgent, AP2183b), mouse anti-CD36 (BD Biosciences, San Jose, CA, USA, 552544), rabbit anti-SR-BI (NOVUS Biologicals, Littleton, CO, USA, NB400113), rabbit anti-CD204 (Abcam, ab151707), TLR4 (Invitrogen, Carlsbad, CA, USA, 48- 2300), goat anti-GAPDH (SICGEN, AB0049-20), goat anti-calnexin (SICGEN, Cantanhede, Portugal, AB0041-20), mouse anti-β-tubulin (Hybridoma Bank, Iowa City, IA, USA, E7). ..

    Article Title: Cholesteryl Hemiazelate Present in Cardiovascular Disease Patients Causes Lysosome Dysfunction in Murine Fibroblasts
    Article Snippet: .. After electrophoresis, proteins were transferred toward an activated PVDF membrane in 1x transfer buffer at 4 °C for 1 h. Membranes were blocked with blocking buffer (5% BSA or milk in 1X TBS-T–Tris-buffered saline, 0.01% tween) for 1 h at room temperature (RT), followed by overnight incubation at 4 °C with the following primary antibodies diluted in blocking buffer: rat anti-LAMP1 (Hybridoma Bank, 1D4B), rabbit anti-CTSD (Abcam, ab75852), rabbit anti-LAL (Abcam, Cambridge, UK, ab154356), mouse anti-P62 (Abgent, San Diego, CA, USA, AP2183b), rabbit anti-LC3B (Cell Signaling, Danvers, MA, USA, 2775S), rabbit anti-phospho-mTOR (Cell Signaling, 5536S), rabbit anti-mTOR (Cell Signaling, 2983S), 4E-BP1 (Cell Signaling, 9644S), phosphor-4E-BP1 (Cell Signaling, 2855S), rabbit anti-S6 ribosomal protein (Cell Signaling, 2217S), rabbit anti-phospho-S6 (Cell Signaling, 4858S), rabbit anti-caspase 3 (Cell Signaling, 9661S), rabbit anti-p16-INK4A (Proteintech, Rosemont, IL, USA, 10883-1-AP), mouse anti-p21 (Santa Cruz, Dallas, Texas, United States, sc-6246), mouse anti-SQSTM1 (Abgent, AP2183b), mouse anti-CD36 (BD Biosciences, San Jose, CA, USA, 552544), rabbit anti-SR-BI (NOVUS Biologicals, Littleton, CO, USA, NB400-113), rabbit anti-CD204 (Abcam, ab151707), TLR4 (Invitrogen, Carlsbad, CA, USA, 48-2300), goat anti-GAPDH (SICGEN, AB0049-20), goat anti-calnexin (SICGEN, Cantanhede, Portugal, AB0041-20), mouse anti-β-tubulin (Hybridoma Bank, Iowa City, IA, USA, E7). ..

    Chromatin Immunoprecipitation:

    Article Title: SR-B1 drives endothelial cell LDL transcytosis via DOCK4 to promote atherosclerosis.
    Article Snippet: .. Reporting for specific materials, systems and methods Materials & experimental systems n/a Involved in the study Unique biological materials Antibodies Eukaryotic cell lines Palaeontology Animals and other organisms Human research participants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Anti-SR-B1 (NB400-104, NB400-113 and NB400-134,Novus; ab52629, Abcam), anti-PDZK1 (ab64856, Abcam), antiLOX-1(MAB1798, R&D Systems), anti-LDLR (AF2148,R&D Systems), anti-CD36 (NB400-144,Novus; MA-5-14112, Invitrogen), antiGAPDH (sc-365062, Santa Cruz), anti-DOCK4 (ab85723, Abcam)a anti-HIS tag (ab18184, Abcam), anti-CD45 (103101, clone 30- F11, biolegend), anti-F4/80 (clone BM8, biolegend), anti-human apoB (MA5-15851, ThermoFisher), anti-calnexin (ADI-SPA-860-F, Enzo Life Sciences), anti-Rac1 (clone 23A8 , Cat.# 05-389, Millipore), and anti-transferrin receptor (ab84036, Abcam). ..

    Flow Cytometry:

    Article Title: SR-B1 drives endothelial cell LDL transcytosis via DOCK4 to promote atherosclerosis.
    Article Snippet: .. Reporting for specific materials, systems and methods Materials & experimental systems n/a Involved in the study Unique biological materials Antibodies Eukaryotic cell lines Palaeontology Animals and other organisms Human research participants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Anti-SR-B1 (NB400-104, NB400-113 and NB400-134,Novus; ab52629, Abcam), anti-PDZK1 (ab64856, Abcam), antiLOX-1(MAB1798, R&D Systems), anti-LDLR (AF2148,R&D Systems), anti-CD36 (NB400-144,Novus; MA-5-14112, Invitrogen), antiGAPDH (sc-365062, Santa Cruz), anti-DOCK4 (ab85723, Abcam)a anti-HIS tag (ab18184, Abcam), anti-CD45 (103101, clone 30- F11, biolegend), anti-F4/80 (clone BM8, biolegend), anti-human apoB (MA5-15851, ThermoFisher), anti-calnexin (ADI-SPA-860-F, Enzo Life Sciences), anti-Rac1 (clone 23A8 , Cat.# 05-389, Millipore), and anti-transferrin receptor (ab84036, Abcam). ..

    Article Title:
    Article Snippet: Anti-mouse CD36 (Polyclonal, R&D Systems, # AF2519, Lot: VYQ0220021): validated by manufacturer for species: mouse; application: western blot, flow cytometry, immunohistochemistry. .. Anti-mouse SR-B1 (Polyclonal, Novus Biologicals, # NB400-104, Lot: U): validated by manufacturer for species: human, mouse, rat; application: western blot, flow cytometry, immunocytochemistry, immunofluorescence, immunohistochemistry. .. Alexa Fluor 488 anti-goat IgG(H+L) (Polyclonal, Invitrogen, # A-11055, Lot: 1771339): validated by manufacturer for species: goat; application: immunohistochemistry, immunocytochemistry, flow cytometry.

    Magnetic Resonance Imaging:

    Article Title: SR-B1 drives endothelial cell LDL transcytosis via DOCK4 to promote atherosclerosis.
    Article Snippet: .. Reporting for specific materials, systems and methods Materials & experimental systems n/a Involved in the study Unique biological materials Antibodies Eukaryotic cell lines Palaeontology Animals and other organisms Human research participants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Anti-SR-B1 (NB400-104, NB400-113 and NB400-134,Novus; ab52629, Abcam), anti-PDZK1 (ab64856, Abcam), antiLOX-1(MAB1798, R&D Systems), anti-LDLR (AF2148,R&D Systems), anti-CD36 (NB400-144,Novus; MA-5-14112, Invitrogen), antiGAPDH (sc-365062, Santa Cruz), anti-DOCK4 (ab85723, Abcam)a anti-HIS tag (ab18184, Abcam), anti-CD45 (103101, clone 30- F11, biolegend), anti-F4/80 (clone BM8, biolegend), anti-human apoB (MA5-15851, ThermoFisher), anti-calnexin (ADI-SPA-860-F, Enzo Life Sciences), anti-Rac1 (clone 23A8 , Cat.# 05-389, Millipore), and anti-transferrin receptor (ab84036, Abcam). ..

    Western Blot:

    Article Title:
    Article Snippet: Anti-mouse CD36 (Polyclonal, R&D Systems, # AF2519, Lot: VYQ0220021): validated by manufacturer for species: mouse; application: western blot, flow cytometry, immunohistochemistry. .. Anti-mouse SR-B1 (Polyclonal, Novus Biologicals, # NB400-104, Lot: U): validated by manufacturer for species: human, mouse, rat; application: western blot, flow cytometry, immunocytochemistry, immunofluorescence, immunohistochemistry. .. Alexa Fluor 488 anti-goat IgG(H+L) (Polyclonal, Invitrogen, # A-11055, Lot: 1771339): validated by manufacturer for species: goat; application: immunohistochemistry, immunocytochemistry, flow cytometry.

    Immunocytochemistry:

    Article Title:
    Article Snippet: Anti-mouse CD36 (Polyclonal, R&D Systems, # AF2519, Lot: VYQ0220021): validated by manufacturer for species: mouse; application: western blot, flow cytometry, immunohistochemistry. .. Anti-mouse SR-B1 (Polyclonal, Novus Biologicals, # NB400-104, Lot: U): validated by manufacturer for species: human, mouse, rat; application: western blot, flow cytometry, immunocytochemistry, immunofluorescence, immunohistochemistry. .. Alexa Fluor 488 anti-goat IgG(H+L) (Polyclonal, Invitrogen, # A-11055, Lot: 1771339): validated by manufacturer for species: goat; application: immunohistochemistry, immunocytochemistry, flow cytometry.

    Immunofluorescence:

    Article Title:
    Article Snippet: Anti-mouse CD36 (Polyclonal, R&D Systems, # AF2519, Lot: VYQ0220021): validated by manufacturer for species: mouse; application: western blot, flow cytometry, immunohistochemistry. .. Anti-mouse SR-B1 (Polyclonal, Novus Biologicals, # NB400-104, Lot: U): validated by manufacturer for species: human, mouse, rat; application: western blot, flow cytometry, immunocytochemistry, immunofluorescence, immunohistochemistry. .. Alexa Fluor 488 anti-goat IgG(H+L) (Polyclonal, Invitrogen, # A-11055, Lot: 1771339): validated by manufacturer for species: goat; application: immunohistochemistry, immunocytochemistry, flow cytometry.

    Immunohistochemistry:

    Article Title:
    Article Snippet: Anti-mouse CD36 (Polyclonal, R&D Systems, # AF2519, Lot: VYQ0220021): validated by manufacturer for species: mouse; application: western blot, flow cytometry, immunohistochemistry. .. Anti-mouse SR-B1 (Polyclonal, Novus Biologicals, # NB400-104, Lot: U): validated by manufacturer for species: human, mouse, rat; application: western blot, flow cytometry, immunocytochemistry, immunofluorescence, immunohistochemistry. .. Alexa Fluor 488 anti-goat IgG(H+L) (Polyclonal, Invitrogen, # A-11055, Lot: 1771339): validated by manufacturer for species: goat; application: immunohistochemistry, immunocytochemistry, flow cytometry.



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